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Metabolic B12 deficiency is prevalent, impacting 10%-40% of the population
The experiment was carried out in three biological replicates on different days, with cells from different passages
Hanefeld M
After resuspending bacterial cells in the lysis buffer supplemented with 10 mM imidazole, proteins were captured with the Ni-NTA affinity chromatography (Sigma-Aldrich) and subjected to SEC in buffer A
6A, B), indicating IMP-366-mediated necroptosis is dependent on ferroptosis
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