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Solo seis aos ms tarde, en 1955, Sanger volvi a marcar un hito al publicar por primera vez la secuencia completa de una protena: la insulina, habiendo logrado secuenciar los 51 aminocidos que conforman las dos cadenas de la insulina, as como determinar la posicin y composicin de los tres puentes disulfuro que las unen y la tcnica desarrollada pas a conocerse como el mtodo de degradacin con DNP (dinitrofenil), sentando las bases para la secuenciacin de protenas