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After full recovery, correct cannulation was tested by observing water drinking responsiveness after infusion of 1 l human angiotensin II (Sigma-Aldrich, A9525) at a concentration of 24 M in artificial cerebrospinal fluid (distilled water with 125 mM NaCl, 2.5 mM KCl, 2.6 mM NaHCO 3 , 1.25 mM NaH 2 PO 4 2H 2 O, 25 mM d-glucose monohydrate, 1 mM MgCl 2 , 2 mM CaCl 2 )

301 , R1755R1764 (2011)

Improper technique can spread infection to deeper tissue planes or the bloodstream

Repa, J.J

HDRS-17, the Hamilton Depression Rating Scale-17

This is true especially when we know very little about a topic and are looking to discover new things that can be further explored