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Blood glucose experiment in db/db mice After the acclimation period (at least 5 days), mice (male db/db mice (Charles River laboratories) 7 weeks old, 40 g) were fasted for 6 h, then blood was collected to measure levels of glucose and insulin

The standard protocol involves weekly subcutaneous injections, starting at a low dose and titrating upward over several weeks

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Metabolomics 14 , 49 (2018)