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Subcutaneous injection with a 2730 gauge needle at 45 is significantly less painful than intramuscular injection, which requires a thicker 2225 gauge needle inserted 90 to penetrate muscle belly

In organotypic hippocampal slice cultures, Dihexa and Nle-AngIV similarly augmented spinogenesis, with control slices showing 7 spines per 50-m dendrite length, compared to 11 for both Dihexa and Nle-AngIV treated slices [2]

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While direct evidence for Dihexa's effects on neurogenesis remains limited, the HGF/c-Met system that Dihexa activates is known to stimulate stem cell differentiation and promote neurogenesis in the adult hippocampus