We observed that n-acetylglutamic acid (arginine precursor) was enriched in T2, whereas cytosine, xanthine, and 7-methylguanine (nucleotide metabolism), together with threosphingosine and 3-methylxanthine (active metabolites) where enriched in T3, whilst hydrocinnamic acid (active metabolite) was depleted
(3) Ultrasonic extraction in an ice-water bath for 10 min, and leave at -40C overnight
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The blend is designed to be complete on its own, and treating it that way is the conservative, research-consistent default
10.1089/ars.2011.4170 Antioxid