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In brief, the gradient started with 5% buffer B and increased stepwise to 45% over 45 min, followed by a wash-out at 95% buffer B, all at a flowrate of 300 nl min 1

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This ensures that microdosing is suitable for your individual circumstances and helps mitigate potential risks associated with unsupervised use

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The addition of alcohol may worsen these issues as its impact on the kidneys impairs the bodys ability to regulate fluid and electrolytes