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Real-time quantitative polymerase chain reaction (RT-qPCR) The samples were added into Trizol for cracking, then chloroform was added for centrifugation to obtain the upper liquid phase, and isopropyl alcohol was added for centrifugation to obtain the precipitate
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however, excessive doses of vitamin C may lead to gastrointestinal discomfort
Source, country, grouping, sample size, age, duration of treatment et al were extracted from the above-included studies
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