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The sorted cells were washed twice with 50 mL phosphate-buffered saline to remove the FreSHtracer and then further cultivated in culture medium for 24 h prior to in vitro or in vivo experiments, as described below

doi:10.1056/NEJMoa1200111 Mingrone G, Panunzi S, De Gaetano A, et al

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Nausea, constipation, fatigue, and digestive discomfort are frequently reported

Cloudiness suggests prior temperature excursions, contaminated water or peptide, or improper reconstitution technique

Similarly, orlistat, which works by blocking fat absorption in the gut, can cause gastrointestinal discomfort when dietary fat intake is too high