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This method inhibits endothelial cell proliferation and angiogenesis, which are connected to proliferative diabetic retinopathy
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The TAC and reduced glutathione assay were performed following the manufacturer's instructions
The membrane was washed three times in Tris-buffered saline with Tween 20 (TBS-T) for 10 min, and treated with HRP-conjugated anti-rabbit or anti-mouse IgG (GE Healthcare) for 1 h
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