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However, under conditions of stress and inhibition of the electron transport chain, the enzyme works in the opposite direction by transferring electrons from NADPH to NAD + , which impairs mitochondrial antioxidant defenses and feeds more reducing equivalents into the impaired electron transport chain leading to increased electron leakage and superoxide formation [133]

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After laboratory preparation for in-vitro work, follow your institution's validated handling procedure and avoid repeated freeze-thaw cycles

Kong N, Tao W, Ling X, Wang J, Xiao Y, Shi S, et al

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