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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

Lu et al., 2022)

Hepatic APAP metabolism results in the production of the reactive metabolite N -acetyl- p -benzoquinone imine (NAPQI), which under physiological conditions is cleared by its conjugation with glutathione (GSH) to prevent its targeting to mitochondria

The mechanism likely involves correction of oxidative stress and modulation of glutamate signaling via the cystine-glutamate antiporter (system Xc-) [73][74]