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Thus, to enable detection of specific mechanism-related interactions, antibiotic doses were selected to inhibit 2030% of growth relative to growth without antibiotics, as measured by OD 600 29,31,32 (Table 1 and Supplementary Fig

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To investigate the transcriptional regulatory effect of Gln on the GGCT gene, a 2000-bp sequence of the GGCT gene promoter region was inserted into the pGL4-Basic vector

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These findings were confirmed in primary hepatocytes taken from mice lacking MPC2 in the liver ( Mpc2 LKO ), when compared to cells from mice with an intact allele ( Mpc2 fl/fl )