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After the membrane was blocked with 5% skim milk TBST (20 mM Tris-Cl (pH 7.4), 150 mM NaCl, 0.1% Tween-20), it was incubated with streptavidin (SA) coupled to horseradish peroxidase (HRP) (1:1000, Pierce) and washed three times with TBST for 10 min

The maintenance dose is not the same for everyonesome maintain at 10 mg, while others may find 15 mg appropriate if well-tolerated

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It's a retrospective registry study

No obstante, el uso de estos medicamentos para bajar de peso tiene sus riesgos

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