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Immunofluorescent staining Wild-type and CTPS1-KO HeLa cells, or H1.4-KO HeLa cell lines stably expressing mutant H1.4 (5 10 4 ) were seeded into a 35-mm glass-bottomed dish and cultured in a humidified incubator at 37 C under 5% CO 2 for 48 h