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This experiment confirmed that for the total reduction of N-Hcy-Lys at 10 min with 250 molar excess of TCEP is required
Glutathione deficiency in the pathogenesis of SARS-CoV-2 infection and its effects upon the host immune response in severe COVID-19 disease
Vitamin C plays an important role to keep glutathione in its reduced form so that it can continue to provide free radical-quenching effects [2-5]
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Importantly, an additional experiment showed that LASSS-treated BSA (after the removal of un-reacted LASSS) exhibited nitration resistance to the optimal peroxynitrite-treatment (1:2000 ratio), as shown in supplementary Figure 2, suggesting a model that LASSS-induced chemical modification mechanism exerts a nitration-inhibitory effect on a wide range of proteins susceptible to tyrosine nitration
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