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Secondary antibodies, Alexa 596 (#A-11012, Thermo Scientific, USA) and Alexa 488 (#A-21202, Thermo Scientific, USA) were used at 1:1000 dilution for double labeling for 30 min at RT

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Sleep quality and tiredness were measured both objectively (using polysomnography) and subjectively (through self-reports)

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- CJC-1295: CJC-1295 is a synthetic analog of growth hormone-releasing hormone (GHRH)