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To assess the half maximal inhibitory concentration (IC50) of FOXO4-DRI on the serum limitation-induced senescent fibroblast and the non-senescent control fibroblast, the fibroblast viability in the 2% FBS induced senescent fibroblast and 10% FBS cultured non-senescent control fibroblast with increasing concentrations of FOXO4-DRI was detected, and the cell viability curves were showed in Supplementary Fig

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RNA isolation and real-time quantitative PCR (RT-qPCR) Total RNA was isolated from cells using a total RNA preparation kit (Axygen, NY, USA) in accordance with the manufacturers protocol

Animal models report accelerated healing of gut, tendon, ligament, and muscle injuries [6]

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