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Cells were washed with PBS, dissociated in 10 mM EDTA (2 ml per well) at 37 C for 5 min, and washed three times in cold PBS
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Life Sciences
Finally, another report proposed the synthesis of PLGA nanocarriers decorated with PEG and glutathione (GSH), for the intracellular delivery of curcumin: the double functionalization resulted in an increased NP uptake by neuronal cells and, in particular, the GSH conjugation modified the NP internalization pathway, avoiding the lysosomal degradation and increasing the therapeutic fate for potential application in the treatment of AD (Paka and Ramassamy, 2017)
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