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The crown was embedded in an acrylic resin block with either buccal or lingual surface exposed, and the exposed surface was flattened with a 600 grit silicon carbide paper to obtain a rough enamel surface
It supports antioxidant protection and helps reduce cell damage from free radicals
After the third wash, the supernatant was discarded, and the erythrocytes were diluted with 1.5 mL of saline solution (0.9% NaCl)
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Prakash, Amitabh, and Karen L
Katz AM
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