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After collection, the samples were centrifuged at 2000 g and 4 C for 10 min, and aliquots were stored at -80 C for later analyses

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Most of these molecules are produced from oxygen in numerous metabolic processes occurring throughout the body, which primarily take place in the mitochondria, endoplasmic reticulum (ER) and peroxisomes

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Six mice were randomly divided into 2 groups and injected into the tail vein with Ru(II)-OH and NP4, respectively, and 10 L of blood was collected from the tail tip at 5 min, 10 min, 20 min, 30 min, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h, and 48 h