Sleep improvements typically appear within 2-4 weeks, while body composition and performance changes develop over 2-3 months, with continued optimization through 6 months
Group I n = 23, group II n = 36
Cytokine levels were measured using enzyme-linked immunosorbent (ELISA) assay kits: Human 1L-10 ELISA Ready-SET-Go from Affymetrix (Cat# 88-7106) and Human TNF- Uncoated ELISA (Cat # 88-7346), Human IFN- Uncoated ELISA (Cat # 88-7316), and Human IL-6 Uncoated ELISA (Cat # 88-7066) from Invitrogen to quantity detection of cytokines
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Signal sensitivity pulsed dosing is the conservative way to avoid blunting the response over time