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If youd like to understand the science behind one-carbon metabolism in more detail, explore our Methylation and Homocysteine guide, which explains how methylation works, why homocysteine is such an important biomarker, and how nutrients support these interconnected pathways
A total of 3.0 ml reaction mixture contained 2.85 ml of 50 mM potassium phosphate buffer (pH 7) and 0.05 ml sample
Baumgartner, R
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After centrifugation at 500g for 15 min (70), semen samples from each group ( n = 3) were collected with the number of frozen-thawed spermatozoa adjusted to a final concentration of 1 10 6 sperms/ml, added to the above fertilization droplet containing MII oocytes (repeated in triplicate, 205 oocytes for the NC group, 210 oocytes for the GSH group, 220 oocytes for the SeNPs group and 217 oocytes for the GSH + SeNPs group, repeated in triplicate) and cultured in a CO 2 incubator (38.5C, 5%CO 2 and 5%O 2 ) for 18 h
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