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If your medication arrives warm, if the cold pack in the package is fully melted, or if your medication sat on a hot porch for hours before you retrieved it, the potency may be compromised

In addition, a tandem-labeled GFP-mRFP-LC3B construct was used to monitor autophagic flux

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Unlike heat exposure, which causes gradual degradation, freezing can destroy a significant percentage of active peptide in a single event

Additionally, exposure to ROS can lead to modifications that cause genomic DNA mutations, negatively affect protein activity, damage cellular membranes, and eventually leading to cell death

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