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The desalted sample was evaporated to dryness in a speedvac and then resuspended in 10 L Buffer A and analyzed by mass-spectrometry using the following LC-MS gradient: 5% buffer B in buffer A from 0-15 min, 515% buffer B from 1517.5 min, 1535% buffer B from 17.592.5 min, 3595% buffer B from 92.595 min, 95% buffer B from 95105 min, 955% buffer B from 105107 min, and 5% buffer B from 107125 min

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Studies have reported that microorganisms transform few inorganic forms of As to organic forms and vice versa by the process of methylation or demethylation ( Arsenicibacter rosenii , has been identified to encode an efficient enzyme for the As methylation and volatilization ( Rhodopseudomonas palustris has been observed to enhance tolerance toward As(III) stress in E