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Both responses are normal and do not indicate a problem with your treatment plan

Use fresh syringes for each injection

Subcutaneous injection delivers the peptide directly into the bloodstream, bypassing digestive degradation making it the preferred route for musculoskeletal or systemic applications

In general, there is an overall assumption that male mice produce the USVs during courtship with some studies suggesting that the female mouse does not vocalize in the presence of an anesthetized male while the converse is true if a male mouse is around an anesthetized female mouse

Protocol : Dosing is typically titrated over several weeks to minimize gastrointestinal discomfort and optimize tolerance

Dahl, D., Onishi, Y., Norwood, P., Huh, R., Bray, R., Patel, H., & Rodrguez,