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Tissues were then washed three times in PBS and homogenized in SDS lysis buffer (167 mM NaCl, 0.01% SDS, 1.1% Triton X-100, 1.2 mM EDTA, 16.7 mM Tris-HCl, pH 8.1) containing PhosSTOP Phosphatase Inhibitor Cocktail (Roche Applied Science, Penzberg, Germany)

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PubMed Chang CH, Tsai WC, Lin MS, et al

For a researcher, this means the three compounds answer different questions