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Cells were seeded in 6-well plates 24 h before transfection, reaching approximately 80% confluency by the time of the procedure
10.1152/physrev.00005.2017 109 Silwal-PanditL.LangerdA.Brresen-DaleA.-L
Then measure carefully using a syringe marked in mL
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Glutamine and Cancer. Journal of Nutrition 131(9 Suppl): 2539S2542S
Western blotting Myocardial tissue was lysed in radioimmunoprecipitation assay buffer (RIPA) lysate (AR0102-30, BOSTER, 30 ml) and ethylenediaminetetraacetic acid (EDTA)-containing protease inhibitor (100 ) buffer before extraction of the protein and was sonicated to fragment the myocardial tissue
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