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In the present study, we: 1) assessed the functional role of endogenous VTA GLP-1 signaling in cocaine seeking using projection-specific chemogenetic approaches, 2) determined cell type-specific expression patterns of GLP-1Rs in the VTA using fluorescent in situ hybridization (FISH) and single nuclei RNA-sequencing (snRNA-seq) methods, and 3) used in vivo fiber photometry in freely moving transgenic rats to further our understanding of the cell type-specific mechanisms in the midbrain underlying the suppressive effects of a GLP-1R agonist on cocaine seeking