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We next used lentiviral vectors to stably express RNAi-resistant GFP-tagged WT ASPL or truncation mutants in G3BP-tdTomato knock-in U2OS cells and used live-cell imaging to assess SG formation in response to HS

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A 5 mg vial reconstituted with 2 mL bacteriostatic water yields a concentration of 2.5 mg/mL or 2500 mcg/mL