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By treating metabolically active adipocytes and hepatocytes with 5-amino-1-methylquinolinium, researchers can precisely inhibit intracellular NNMT activity, monitor changes in the nicotinamide pathway and NAD+ levels, and analyze effects on energy metabolism

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The treatment group showed significant reductions in body weight (approximately 5% loss) and white adipose tissue mass (35% reduction) compared to control animals, despite no changes in food intake

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