Efficiency of mRNA depletion was assessed 72 h post-transfection using qPCR or western blot in the case of ACSL1 (Cell Signaling, Antibody #4047, used 1:1000), GPX4 (Abcam, [EPNCIR144], ab125066, used 1:1000), DGAT1 (Abcam, [EPR13430-4] - N-terminal ab181180, used 1:5000), DGAT2 (ThermoFisher Scientific, PA5-103785, used 1:1000), and ATGL (Cell Signaling, Antibody #2138, used 1:1000)
This pathway involves activation of phosphatidylinositol phospholipase C (PI-PLC), phosphatidylinositol 4,5-bisphosphate (PIP 2 ) and inositol trisphosphate (IP 3 ), ultimately resulting in mobilization of intracellular Ca 2+ stores (Camia et al., 2007)
Argelaguet R, Arnol D, Bredikhin D, et al
Moisture-Retentive Barrier Care: Formulated with a pH-balanced, non-stripping base that preserves the skin's essential moisture layer, leaving the skin feeling exceptionally soft and hydrated without any tight or dry sensation
The importance of the fatty acid transporter L-carnitine in non-alcoholic fatty liver disease (NAFLD)
Oxidative damage and antioxidant defense in ferroptosis