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The next day, after three washes in TBST, the membranes were incubated with secondary antibodies, including an HRP-conjugated anti-rabbit secondary antibody (Proteintech, SA00001-2, 1:5000) and anti-mouse secondary antibody (Proteintech, SA00001-1, 1:5000) at room temperature for 1 h

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It is essential to perform a visual check before administering the drug