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Was the human material fetal fibroblasts in a dish
Trypsin activity was quenched by adding 200 L of MEF medium (10% FBS in DMEM), and the cell suspensions were centrifuged at 300 g
The main conclusion here, that excess SAM is catabolized to MTA and adenine, leading to methylation deficiencies, stands
Validating this biological reality is the essential first step toward meaningful, compassionate management and recovery
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