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After blocking in 5% goat serum (Beyotime) for 30 min, the sections were incubated with antibodies against insulin, glucagon, fibronectin, desmin, and -SMA (Supplementary Table 2) at 4 C overnight
Potentially leading to bile becoming stagnant
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The medication takes 4 to 5 weeks to reach steady-state blood levels, which is why the dose titration schedule starts low and increases gradually
Gravimetric analysis for weight verification Weighing the actual peptide content requires careful technique