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There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes

This lower dose sustains the benefits achieved during optimization while reducing the total peptide load on your system

However, the relationship between the two is even more interesting than a simple "contains" or "doesn't contain" label

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Flow cytometry studies of intracellular adriamycin in single cells in vitro