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Mozafari M, Nekooeian AA, Panjeshahin MR, et al

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Tear fluid was collected from 60 participants, cognitively normal (CN) individuals ( n = 32), patients with mild cognitive impairment ( n = 14), and dementia ( n = 14)

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The method for synthesizing semaglutide as claimed in claim 1, wherein said step (2) comprises coupling Fmoc-Gly-OH to a solid-phase resin containing an ionic liquid Linker, specifically coupling amino acids using Fmoc-Gly-OH and the solid-phase resin containing the ionic liquid Linker