coli WT, dsbA , gshA or dsbA gshA cells producing either roGFP2 or roGFP-iL from pPT plasmids cultivated as described above were harvested, washed and resuspended to an OD 600 of 1.0 in PBS buffer (1.5 mM KH 2 PO 4 , NaCl 150 mM, 2.7 mM Na 2 HPO 4 -7xH 2 O, pH 7.4)
Kinetic data were analysed in Excel and SigmaPlot 12 by non-linear and linear regression according to MichaelisMenten, LineweaverBurk, EadieHofstee and Hanes theory 11,12,18
doi: 10.1111/jnc.15519 11 WangZLYuanLLiWLiJY
R., Yu, Q., Yamamoto, K., Encarnacin-Rosado, J., Rose, R
Mitochondrial membrane properties, controlled by the fatty acid composition and cholesterol/phospholipid molar ratio, also affect GSH transport
This review delineates the molecular and cellular pathways by which Hg exerts neurotoxic effects, emphasizing its disruption of the glutamateglutamineGABA cycle and the resulting excitotoxic activation of NMDA receptors