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The genuine human Phase II trials used full-length thymosin beta-4 as a topical eye drop for dry eye, and even those had mixed results on their primary endpoints

doi:10.1007/s00253-001-0902-7

Patch Testing: For topical research, conducting a patch test on a small, inconspicuous area of skin (e.g., the inner forearm) is always a recommended step to assess individual skin compatibility before broader application

Characterization of a tool compound for pre-clinical studies of amylin analogs such as cagrilintide

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